DNase I, RNase-free is an endodeoxyribonuclease that catalyzes to the same extent the degradation of both single- and double-stranded DNA randomly, and produces 5'-P terminal oligonucleotides.

       The activity of DNase I is dependent on Ca²⁺, and can be activated by bivalent metal ions Mg²⁺, Mn²⁺, etc. In the presence of Mg²⁺, the enzyme can randomly recognize and cut any site on any strand of double-stranded DNA. In the presence of Mn²⁺, nearly identical sites on the two strands of DNA can be recognized and cut, resulting in flat ends or sticky end DNA fragments with 1 to 2 nucleotides overhang.

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