GIII Visual All-in-One MasterMix (with dsDNase) is a high-quality, efficient, and convenient one-step cDNA synthesis kit. It is designed to minimize contamination and contains all the necessary components for first-strand cDNA synthesis, including thermostable M-MLV GIII Reverse Transcriptase and its reaction buffer, RNase inhibitor, dNTPs, Oligo(dT)20VN, and random primers-all the necessary components. Simply add RNA template and water to initiate the reaction. Compared to conventional reverse transcription mixes, this product eliminate pipetting errors with non-interfering, visible tracking dye. The cDNA obtained using this kit is primarily used for downstream qPCR experiments.
       RNA extracted from tissues and cells often contains genomic DNA contamination. If it is not removed prior to reverse transcription, both genomic DNA and cDNA can be amplified during subsequent qPCR reactions (especially when the primers are designed within the same exon), which can affect the accuracy of gene expression quantification. This kit employs dsDNase to efficiently remove genomic DNA contamination. DsDNase specifically digests double-stranded DNA (dsDNA) or DNA strand in DNA-RNA hybrid chains. It is heat-labile and can be rapidly and irreversibly inactivated at the reverse transcription temperature. Compared to traditional methods that use DNase I to remove genomic DNA contamination, dsDNase does not require the addition of EDTA for inactivation. This not only saves experimental time but also reduces the  inhibition of reverse transcription.
       Two protocols can be choosen based on the content of genomic DNA contamination: two steps that separate genomic DNA removal and reverse transcription, or one-step method that combines genomic DNA removal and reverse transcription.

Store at -20℃ for 2 years.