Cell-Free Protein Synthesis Kit is an extract-based transcription/translation system derived from E. coli cells. It enables the site-specific incorporation of the Nε-crotonyl-L-lysine (Kcr) into the target protein by utilizing the amber stop codon TAG in the DNA sequence. The kit contains three additional components: the noncanonical  amino acid Kcr, tRNA, and aminoacyl-tRNA synthetase. Dosage can be optimized according to requirements.
Note: This product under this catalog number enables the expression of disulfide bond-rich proteins.
 

Store at -80℃. Valid for 12 months.
Dry ice transportation. After opening, all components should be stored at -80℃.
To avoid multiple freeze-thaw cycles, Solution A and Solution B can be aliquoted according to reaction volume, snap-frozen in liquid nitrogen, and then stored at -80℃.