Restriction endonucleases (Restriction enzymes) are a class of endonuclease that can recognize specific nucleotide sequences (4~8 base pairs) within double-stranded DNA and cleave DNA at specific sites. The key characteristic of restriction enzymes is their ability to accurately cut DNA and generate defined ends. The LightNing® restriction endonucleases provided by BestEnzymes use only one universal reaction buffer, which provide a better experience for both single and double enzyme digestions. Moreover, LightNing® restriction endonucleases are compatible with the buffers used in downstream experiments, achieving true one-tube convenience. The LightNing® restriction enzymes can complete digestion with 5~15 minutes, which not only saves user's time but also significantly reduces star activity.

Real-time fluorescence quantitative PCR (qPCR) technology utilizes fluorescent dyes or fluorescently labeled specific probes to track and monitor the PCR products in real-time. We have developed multiple qPCR products, among which the most representative one is Taq SYBR® Green qPCR Premix, with its core component being antibody-modified hot-start Taq DNA polymerase. Along with optimized buffer, it ensures high efficiency and specificity of amplification, enabling accurate quantification of a wide range of template concentrations and obtaining stable and reliable qPCR results. Additionally, the Universal version contain a special reference dye compatible with almost all qPCR instruments, eliminating the need to add ROX reference dye on different qPCR instruments.

The core component of these products is the high-performance M-MLV GIII Reverse Transcriptase, which has independent intellectual property rights. It exhibits excellent advantages in terms of thermal stability, continuous synthesis capability, cDNA yield, and inhibitor tolerance. The All-in-One First-Strand Synthesis MasterMix (with dsDNase) is an efficient, convenient, and contamination-reducing first-strand cDNA synthesis kit. The premix contains all reverse transcription reaction components except for the RNA template. It is easy to use and suitable for fluorescence quantitative PCR analysis of the generated cDNA products. On the other hand, the RTase III Primer Flexible All-in-One Mix provides primers separately from other components, allowing customers to flexibly use different types of primers according to their experimental designs, and is more suitable for full-length cDNA cloning.

It relies on the recombination of inserted fragments with the homologous sequences of 15~25 nt at the ends of the linearized vector. In theory, it allows for the cloning of inserted fragments at any desired position in any vector. It offers advantages such as high cloning efficiency, the ability to simultaneously insert multiple fragments, and rapid reactions.

Buffer solution is usually a solution composed of a weak acid and its conjugate base or a weak base and its conjugate acid buffer pair. It can slow down the change of pH when a certain amount of other substances are added. BMS RTU (Ready-to-Use) instant granules present the commonly used buffers in daily laboratory work in the form of instant granules, eliminating the boring process of preparing buffers, and obtaining stable buffer solutions through simple operations!