PRODUCT
BsaI, GMP Grade
- GMP501S
5'...G G T C T C (N)₁ ↓...3'
3'...C C A G A G (N)₅ ↑...5'
Description
BsaI, GMP Grade, is obtained by recombinant expression in Escherichia coli and can precisely digest target DNA within 15 minutes to 1 hour. This product is manufactured and quality-controlled in compliance with GMP specifications, ensuring full traceability of the production process and raw materials. The entire production process does not involve the use of antibiotics or any animal-derived materials and excipients. Stringent controls are implemented for process-related impurities including host proteins, exogenous DNA, non-specific endonucleases, DNase, RNase, as well as microbial limits and bacterial endotoxins. This product meets the requirements for raw materials in fields such as vaccine and pharmaceutical production.
Components
Component |
Amount |
BsaI, GMP Grade (20 U/μl) |
1 ml |
10× Cut Buffer F, GMP Grade |
6×1 ml |
Features and Usage
• Excellent digest efficiency, handle with complex samples easily
• Accorded with the quality request in Pharmacopoeia, no residual impurities
• Comply with GMP production specifications, animal component free
Heat Inactivation
Incubation at 80℃ for 20 minutes.
Methylation sensitivity
Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by the CpG methylase.
Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by the Dcm methylase.
Storage Condition
Store at -20 ± 5℃ , Valid for 24 months. Transport at ≤ 0℃.