中文

I-SceI

REF No.:
  • EG25504S
Specs:
250 U
Price:
$42
Cut site:
TAGGGATAACAGGGTAAT

5'...T A G G G A T A A↓C A G G G T A A T...3'
3'... A T C C C↑T A T T G T C C C A T T A ...5'

Description

       I-SceI is an intron-encoded endonuclease derived from a group II intron of Saccharomyces cerevisiae. It specifically recognizes and cleaves an 18 bp non-palindromic sequence (5'-TAGGGATAACAGGGTAAT-3'), forming a 3' overhanging sticky end with a length of 4 bp.
 

Components

Component

Amount

I-SceI (5 U/μl)

50 μl

pUC-I-SceI (100 ng/μl) 20 μl
10× CutOne® Buffer 1 ml

10× CutOne® Color Buffer

1 ml

Features and Usage

Recommended Reaction Conditions

1× CutOne® Buffer;
Incubate at 37℃;
Refer to "Protocol for DNA Digestion" for reaction setup.

 

Heat Inactivation

Incubation at 80℃ for 20 minutes.

Storage Condition

-20℃

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