PRODUCT
I-CeuI
- EG25529S
5'...T A A C T A T A A C G G T C C T A A↓G G T A G C G A A...3'
3'...A T T G A T A T T G C C A G↑G A T T C C A T C G C T T...5'
Description
I-CeuI is a homing endonuclease derived from Chlamydomonas eugametos, obtained by recombinant expression and purification in Escherichia coli. I-CeuI specifically recognizes and cleaves a 27‑bp non‑palindromic sequence: 5'-TAACTATAACGGTCCTAAGGTAGCGAA-3',
generating a 4‑bp 3' overhang. Compared with I‑SceI, I‑CeuI has a longer recognition sequence, so its natural occurrence frequency in the genome is much lower (approximately once per 5.6×1017 random bases), resulting in higher specificity for gene editing applications.
Components
|
Component |
Amount |
|
I-CeuI (5 U/μl) |
50 μl |
|
pUC-HE (100 ng/μl) |
20 μl |
|
10× CutOne® Buffer |
1 ml |
|
10× CutOne® Color Buffer |
1 ml |
Features and Usage
Recommended Reaction Conditions
1× CutOne® Buffer；
Incubate at 37℃；
Refer to "Protocol for DNA Digestion" for reaction setup.
Heat Inactivation
Incubation at 80℃ for 20 minutes.
Storage Condition
Store at -20℃ for 2 years.
