PRODUCT
BsiWI
REF No.：
- EG24506S
Specs：
300 U
Price：
$42
Isoschizomers：
PspLI, Pfl23II
Cut site：
CGTACG
5'...C ↓ G T A C G...3'
3'...G C A T G ↑ C...5'
5'...C ↓ G T A C G...3'
3'...G C A T G ↑ C...5'
Description
BsiWI is a Type IIP restriction enzyme that recognizes palindromic sequences. The reaction buffer has been optimized to maximize the functionality of BsiWI. Additionally, the reaction buffer contains recombinant albumin, which enhances the stability of various enzymes.
Components
|
Component |
Amount |
|
BsiWI (10 U/μl) |
30 μl |
|
10× Cut Buffer C |
1 ml |
Features and Usage
Recommended Reaction Conditions
1× Cut Buffer C；
Incubate at 55℃；
Refer to "Protocol for DNA Digestion" for reaction setup.
Heat Inactivation
Incubation at 80℃ for 20 minutes.
Methylation sensitivity
Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by the CpG methylase.
Storage Condition
-20℃
