XmnI is a Type IIP restriction enzyme derived from Xanthomonas manihotis. It was purified following recombinant expression in Escherichia coli. XmnI recognizes and cleaves the sequence GAANN/NNTTC, generating blunt ends. Its optimal activity is observed in Cut Buffer C with star activity control. XmnI is compatible with the universal CutOne® Buffer, allowing convenient double‑digestion with other endonucleases, but prolonged incubations should be avoided to prevent star activity.

Recommended Reaction Conditions

1× Cut Buffer C；
Incubate at 37℃；
Refer to "Protocol for DNA Digestion" for reaction setup.

Heat Inactivation

Incubation at 80℃ for 20 minutes.

Methylation sensitivity

Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by the CpG methylase.

Store at -20℃ for 2 years.