BpiI is a Type IIS restriction enzyme. It specifically recognizes the GAAGAC sequence, cleaves downstream, and generates 5' overhangs with 4 bases. It is commonly used in Golden Gate assembly. BpiI belongs to the non-LightNing® restriction enzymes; however, it is compatible with CutOne® buffer, allowing for double digestion with other LightNing® restriction enzymes. It is one of the commonly used enzymes for Golden Gate assembly. It is applicable for scenarios such as routine cloning or restriction digestion and identification other than Golden Gate assembly.
 

Recommended Reaction Conditions

1× CutOne® Buffer；
Incubate at 37℃；
Refer to "Protocol for DNA Digestion" for reaction setup.

Heat Inactivation

Incubation at 80℃ for 20 minutes.

Store at -20℃ for 2 years.