AarI is an isoschizomer of PaqCI. Type IIS restriction enzyme which recognizes the sequence CACCTGC and cleaves outside of their recognition sequence, generating 4-base 5' overhangs. Especially, AarI requires two or more sites for digestion. AarI Activator supplied to improve cleavage. It recognizes a 7-basesequence that occurs infrequently in genes, is particularly suitable for Golden Gate assembly.
 

Recommended Reaction Conditions

1× CutOne® Buffer;
Incubate at 75℃;
Refer to "Protocol for DNA Digestion" for reaction setup.

Heat Inactivation

Incubation at 80℃ for 20 minutes.

Methylation sensitivity

Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by the CpG methylase.

Store at -20℃ for 2 years.