Tris- Glycine(hereinafter referred to as TG) appears as white to pale yellow instant granules . Each pouch makes  1  L of  1 × TG , which is easy to operate and easy to use. The main components of TG are Tris and Glycine. The concentration of Tris in 1 × TG is 25 mM Tris and  the concentration of glycine in 1 × TG is 192 mM.
       ①TG buffer is commonly used for rapid western blotting after being configured in 20% ethanol for 1×, mainly for wet turning.
       ②For the preparation of non-denaturing protein polyacrylamide gel electrophoresis buffer.

Note: pH is 8.3±0.2@25℃ when made to 1× solution.

1. Put magnetic stirring beads and ~600 ml distilled water into a beaker.
2. While stirring, slowly pour the whole contents from 1 pouch of TG into the beaker; wait until everything is dissolved.
3. Add distilled water to bring the volume to 1 L and 1× solution is made.

Room temperature