中文

M-MLV GIV Reverse Transcriptase

REF No.:
  • EG24101S
Specs:
10000 U
Price:
$150

Description

       M-MLV GIV Reverse Transcriptase is a fourth-generation M-MLV reverse transcriptase obtained through genetic modification and recombination technologies. Compared with the wild-type M-MLV reverse transcriptase, it lacks RNase H activity. Key enhancements include superior thermal stability, with an optimal reaction temperature of 55℃ and sustained 50% activity at 70℃. Furthermore, it demonstrates improved tolerance to complex secondary structures , accelerated reaction speed (completing reactions in as little as 10 minutes), and high template affinity. These combined properties ensure robust performance with low-copy templates and enable efficient synthesis of full-length cDNA fragments up to 20 kb.
 

Components

Component

Amount

M-MLV GIV Reverse Transcriptase (200 U/μl)

50 μl

5× M-MLV First Strand Buffer

500 μl

0.1 M DTT

100 μl

Storage Condition

Store at -20℃ for 2 years.

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