PRODUCT
Description
M-MLV GIV Reverse Transcriptase is a fourth-generation M-MLV reverse transcriptase obtained through genetic modification and recombination technologies. Compared with the wild-type M-MLV reverse transcriptase, it lacks RNase H activity. Key enhancements include superior thermal stability, with an optimal reaction temperature of 55℃ and sustained 50% activity at 70℃. Furthermore, it demonstrates improved tolerance to complex secondary structures , accelerated reaction speed (completing reactions in as little as 10 minutes), and high template affinity. These combined properties ensure robust performance with low-copy templates and enable efficient synthesis of full-length cDNA fragments up to 20 kb.
Components
|
Component |
Amount |
|
M-MLV GIV Reverse Transcriptase (200 U/μl) |
50 μl |
|
5× M-MLV First Strand Buffer |
500 μl |
|
0.1 M DTT |
100 μl |
Storage Condition
Store at -20℃ for 2 years.

