Taq-antibody (B8) is a monoclonal antibody of murine origin that binds Taq polymerase and inhibits its activity efficiently. This antibody is able to bind to Taq DNA Polymerase so strongly that inhibits 95% activity of Polymerase at 55℃. Therefore, it can effectively suppress the degradation of mismatched probes or other materials at low temperatures, reducing non-specific signals. After heated at 95℃for 30 seconds, the enzyme-antibody complex dissociates, and the Taq-antibody (B8) becomes nonfunctional. Once the antibody is denatured, the activity of the Taq DNA polymerase is restored and the enzyme functions normally during the PCR reaction. This product is especially suitable for Quantitative Real-Time PCR based on probe method.
       Exonuclease activity-blocking antibodies and polymerase activity-blocking antibodies can be used in combination to obtain double-blocking antibodies. When these antibodies bind to Taq DNA polymerase, they can simultaneously inhibit both polymerase and exonuclease activities,, further improving the specificity of the reaction.
 

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