The T7 High Yield RNA Synthesis Kit is designed for the large-scale synthesis of RNA through in vitro transcription using T7 RNA polymerase and DNA templates containing a T7 promoter. It is suitable for both long and short RNA transcripts. The T7 Enzyme Mix included RNase inhibitor and inorganic pyrophosphatase. Additionally, DNase I, RNase-free, is provided to eliminate template DNA after the transcription reaction. Using this kit, 1 μg of linearized double-stranded DNA template can yield at least 150 μg of RNA. The synthesized RNA is applicable for a wide range of downstream applications, including in vitro translation, RNA structure and function studies, RNase protection assays, probe hybridization, RNA interference, and more.

-20℃