Poly(A) Polymerase is a polymerase derived from Escherichia coli, also known as E.coli PAP, PAP enzyme, Poly(A) tailing enzyme, or PolyA tailing enzyme. Poly(A) Polymerase catalyzes the rapid and efficient addition of a poly(A) tail to the 3' end of single-stranded RNA (ssRNA) in the presence of ATP. It can use various single-stranded RNA as substrate, while double-stranded RNA and excessively short oligonucleotides (<20 nt) are not recommended as substrates. DNA cannot be used as substrates for this enzyme. Poly(A) tailing reaction catalyzed by E. coli Poly(A) Polymerase can only use ATP, but not ADP or dATP. In addition, when CTP and UTP are used, the incorporation amount is less than 5% of that when using ATP. GTP can not be used by this enzyme.
 

1. poly(A) tailing of RNA for cloning or affinity purification.
2. Enhances translation of RNA transferred into eukaryotic cells.
3. 3' labeling of RNA.
4. cDNA synthesis, add poly(A) tails to miRNA, followed by reverse transcription to obtain cDNA.
 

Store at -20℃ for 2 years.