PRODUCT
Description
T5 Exonuclease is a nucleic acid exonuclease that digests DNA from the 5' end in the 5'→3' direction for both double-stranded and single-stranded DNA. It is capable of digesting DNA at nicks or gaps in linear or circular double-stranded DNA,however, it cannot digest supercoiled DNA. In addition, when the Mg²⁺ concentration in the solution is below 1 mM, the activity of T5 Exonuclease towards single-stranded DNA is inhibited. Consequently, T5 Exonuclease is particularly suitable for degrading linearized and nicked plasmids, removing non-circular DNA from ligation products, seamless cloning (Gibson Assembly), and other applications.
Components
Component |
Amount |
T5 Exonuclease (10 U/μl) |
100 μl |
10× T5 Reaction Buffer |
1 ml |
Storage Condition
-20℃