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Thermostable RNase HII

REF No.:
  • EG24212S
Specs:
200 U
Price:
$110

Description

       This product is derived from the extremely thermophilic bacterium Pyrococcus abyssi (P.abyssi) and obtained through recombinant expression and purification. Thermostable RNase H is an endonuclease that specifically recognizes and cleaves the RNA strand in RNA/DNA heteroduplexes, without cleaving sSDNA, dsDNA, and exhibits minimal cleavage activity towards single-stranded RNA. Thermostable RNase Hll cleaves at the 5' end of ribonucleotide residues, generating a 5'-phosphate group and a 3'-hydroxyl group after cleavage. This Thermostable RNase Hll exhibits optimal activity within the temperature ranging from 60 to 80℃, remains active between 37℃ and 95℃, and exhibits low activity at room temperature. The product is extremely thermostable, with almost no activity loss following incubation at 95℃ for 15 minutes, making it compatible with various PCR reaction systems.
 

Components

Component

Amount

Thermostable RNase HII (2 U/μl)

200 μl

HII Dilution Buffer

2×1 ml

10× RHII Buffer

1 ml

Storage Condition

 -20℃

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