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Cell-Free Protein Synthesis Kit

REF No.:
  • EG24301S
  • EG24301M
Specs:
20 rxns
Price:
$100
Specs:
100 rxns
Price:
$400

Description

       The Cell-Free Protein Synthesis Kit is a product for in vitro protein synthesis based on E. coli lysate. Coupled transcription/translation systems contain an RNA polymerase and the necessary cellular machinery needed to direct protein synthesis (e.g., ribosomes, translation factors and tRNAs). Supplements such as amino acids, an energy source and NTPs complete the system. Proteins are expressed using DNA or RNA as templates. Cell-free protein expression, independent of viable cells, enables rapid, flexible and high-yield protein expression, with numerous advantages over traditional recombinant expression systems:
       1. Time saving. Express target proteins in 1~2 hours, with peak yield achieved in 8~24 hours.
       2. High protein expression level, up to more than 3 mg/ml.
       3. Easy operation, Add DNA or RNA templates to the reaction system, and the reaction can be performed using 96-well plates or centrifuge tubes.

Note: This product is not suitable for the soluble expression of disulfide-rich proteins, as it does not provide the oxidative environment required for correct disulfide bond folding. If needed, please contact us for other REF NO..
 

Components

Component

EG24301S

EG24301M

Cell-free system solution A

300 μl

1.5 ml

Cell-free system solution B

600 μl

3 ml

CFPS-Control Plasmid

2 μg

2 μg

Storage Condition

Store at -80℃. Valid for 12 months.
Dry ice transportation. After opening, all components should be stored at -80℃.

To avoid multiple freeze-thaw cycles, Solution A and Solution B can be aliquoted according to reaction volume, snap-frozen in liquid nitrogen, and then stored at -80℃.

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