The Cell-Free Protein Synthesis Kit is a product for in vitro protein synthesis based on E. coli lysate. Coupled transcription/translation systems contain an RNA polymerase and the necessary cellular machinery needed to direct protein synthesis (e.g., ribosomes, translation factors and tRNAs). Supplements such as amino acids, an energy source and NTPs complete the system. Proteins are expressed using DNA or RNA as templates. Cell-free protein expression, independent of viable cells, enables rapid, flexible and high-yield protein expression, with numerous advantages over traditional recombinant expression systems：
       1. Time saving. Express target proteins in 1~2 hours, with peak yield achieved in 8~24 hours.
       2. High protein expression level, up to more than 3 mg/ml.
       3. Easy operation, Add DNA or RNA templates to the reaction system, and the reaction can be performed using 96-well plates or centrifuge tubes.
Note: The product contains chaperone proteins, which is suitable for expressing insoluble protein.
 

Store at -80℃. Valid for 12 months.
Dry ice transportation. After opening, all components should be stored at -80℃.

To avoid multiple freeze-thaw cycles, Solution A and Solution B can be aliquoted according to reaction volume, snap-frozen in liquid nitrogen, and then stored at -80℃.