PRODUCT
Cell-Free Protein Synthesis Kit (pAcF)
- EG25330S
Description
Cell-Free Protein Synthesis Kit is an extract-based transcription/translation system derived from E. coli cells. It enables the site-specific incorporation of the noncanonical amino acid p-acetyl-L-phenylalanine (pAcF) into the target sequence by utilizing the amber stop codon TAG in the DNA sequence. The kit contains three additional components: the noncanonical amino acid pAcF, tRNA, and aminoacyl-tRNA synthetase. Dosage can be optimized according to requirements.
Note: This product is not suitable for expression of disulfide-rich proteins. If needed, please contact us for other REF NO..
Components
Component |
Amount |
Cell-free system solution A
|
300 μl
|
Cell-free system solution B
|
600 μl
|
CFPS-Control Plasmid
|
2 μg
|
Noncanonical amino acid pAcF (100×)
|
50 μl
|
tRNA (25×)
|
100 μl
|
Aminoacyl-tRNA synthetase (50×)
|
50 μl
|
Storage Condition
Store at -80℃. Valid for 12 months.
Dry ice transportation. After opening, all components should be stored at -80℃.
To avoid multiple freeze-thaw cycles, Solution A and Solution B can be aliquoted according to reaction volume, snap-frozen in liquid nitrogen, and then stored at -80℃.