PRODUCT
Cell-Free Protein Synthesis Kit (pAcF)
- EG25330S
Description
       Cell-Free Protein Synthesis Kit is an extract-based transcription/translation system derived from E. coli cells. It enables the site-specific incorporation of the noncanonical amino acid p-acetyl-L-phenylalanine (pAcF) into the target sequence by utilizing the amber stop codon TAG in the DNA sequence. The kit contains three additional components: the noncanonical  amino acid pAcF, tRNA, and aminoacyl-tRNA synthetase. Dosage can be optimized according to requirements.
Note: This product is not suitable for expression of disulfide-rich proteins. If needed, please contact us for other REF NO..
Components
| Component | Amount | 
| 
Cell-free system solution A | 
300 μl | 
| 
Cell-free system solution B | 
600 μl | 
| 
CFPS-Control Plasmid | 
2 μg | 
| 
Noncanonical amino acid pAcF (100×) | 
50 μl | 
| 
tRNA (25×) | 
100 μl | 
| 
Aminoacyl-tRNA synthetase (50×) | 
50 μl | 
Storage Condition
Store at -80℃. Valid for 12 months.
Dry ice transportation. After opening, all components should be stored at -80℃.
To avoid multiple freeze-thaw cycles, Solution A and Solution B can be aliquoted according to reaction volume, snap-frozen in liquid nitrogen, and then stored at -80℃.


