PRODUCT
I-SceI
REF No.：
- EG25504S
Specs：
250 U
Price：
$42
Cut site：
TAGGGATAACAGGGTAAT
5'...T A G G G A T A A↓C A G G G T A A T...3'
3'... A T C C C↑T A T T G T C C C A T T A ...5'
5'...T A G G G A T A A↓C A G G G T A A T...3'
3'... A T C C C↑T A T T G T C C C A T T A ...5'
Description
I-SceI is an intron-encoded endonuclease derived from a group II intron of Saccharomyces cerevisiae. It specifically recognizes and cleaves an 18 bp non-palindromic sequence (5'-TAGGGATAACAGGGTAAT-3'), forming a 3' overhanging sticky end with a length of 4 bp.
Components
|
Component |
Amount |
|
I-SceI (5 U/μl) |
50 μl |
| pUC-I-SceI (100 ng/μl) | 20 μl |
| 10× CutOne® Buffer | 1 ml |
|
10× CutOne® Color Buffer |
1 ml |
Features and Usage
Recommended Reaction Conditions
1× CutOne® Buffer；
Incubate at 37℃；
Refer to "Protocol for DNA Digestion" for reaction setup.
Heat Inactivation
Incubation at 80℃ for 20 minutes.
Storage Condition
-20℃
