PRODUCT
Description
T4 Polynucleotide Kinase (T4 PNK) is a polyribonucleotide 5'-hydroxyl kinase that catalyzes the transfer of the γ-phosphate group from ATP to the 5'-hydroxyl terminus of oligonucleotide chains, whether they are double-stranded or single-stranded DNA or RNA. Notably, this reaction is reversible. In addition, T4 PNK exhibits 3'-phosphatase activity, which hydrolyzes the 3'-phosphate group from the 3'-phosphate terminus of oligonucleotides, deoxy-3'-monophosphate nucleotides, and deoxy-3'-diphosphate nucleotides.
Components
|
Component |
Amount |
|
T4 Polynucleotide Kinase (10 U/μl) |
50 μl |
|
10× T4 PNK Buffer |
1 ml |
Storage Condition
-20℃
