PRODUCT
Nt.BspQI
REF No.：
- EG23512S
Specs：
2000 U
Price：
$158
Cut site：
GCTCTTCN
5'...G C T C T T C N↓...3'
3'...C G A G A A G N ...5'
5'...G C T C T T C N↓...3'
3'...C G A G A A G N ...5'
Description
Nt.BspQI is a nicking endonuclease that cuts only one strand of the dsDNA substrate; it introduces a nick into the dsDNA substrate without cleaving the dsDNA.
Components
|
Component |
Amount |
|
Nt.BspQI (10 U/μl) |
200 μl |
|
10× Cut Buffer C |
2×1 ml |
Features and Usage
Recommended Reaction Conditions
1× Cut Buffer C；
Incubate at 50℃；
Refer to "Protocol for DNA Digestion" for reaction setup.
This product has 75% activity when performing enzymatic digestion
reactions at 37℃ .
Heat Inactivation
Incubation at 80℃ for 20 minutes.
Storage Condition
-20℃
