PRODUCT
Nt.BstNBI
REF No.：
- EG24504S
Specs：
2000 U
Price：
$158
Cut site：
GAGTCNNNNN
5'...G A G T C N N N N N↓...3'
3'...C T C A G N N N N N ...5'
5'...G A G T C N N N N N↓...3'
3'...C T C A G N N N N N ...5'
Description
Nt.BstNBI is a nicking endonuclease that only cuts one strand of the dsDNA substrate, resulting in nicks on the dsDNA without complete cleavage.
Components
|
Component |
Amount |
|
Nt.BstNBI (10 U/μl) |
200 μl |
|
10× Cut Buffer C |
2×1 ml |
Features and Usage
Recommended Reaction Conditions
1× Cut Buffer C；
Incubate at 55℃；
Refer to "Protocol for DNA Digestion" for reaction setup.
This product has 50% activity when performing enzymatic digestion
reactions at 37℃ .
Heat Inactivation
Incubation at 80℃ for 20 minutes.
Storage Condition
-20℃
