Nb.BbvCI is a nicking endonuclease that cleaves only one strand of DNA on a double-stranded DNA substrate. It creates nicks on dsDNA substrates without cutting through the dsDNA. Nb.BbvCI is commonly used in isothermal nucleic acid amplification (e.g., Strand Displacement Amplification [SDA], Rolling Circle Amplification [RCA]). DNA nicks generated by Nb.BbvCI trigger the strand displacement reaction of DNA polymerase, and the repeated processes of cleavage, displacement, and extension enable exponential nucleic acid amplification.

Recommended Reaction Conditions

1× CutOne® Buffer;
Incubate at 37℃;
Refer to "Protocol for DNA Digestion" for reaction setup.

Heat Inactivation

Incubation at 80℃ for 20 minutes.

Methylation sensitivity

Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by the EcoBI methylase.

Store at -20℃ for 2 years.