PRODUCT
T4 RNA Ligase 1
- EG25204S
- EG25204M
Description
T4 RNA Ligase 1 is an ATP-dependent ligase derived from bacteriophage T4. It catalyzes the ligation of a 5' phosphoryl-terminated nucleic acid donor to a 3 hydroxyl-terminated nucleic acid acceptor through the formation of a 3'→5' phosphodiester bond with hydrolysis of ATP to AMP and PPi. The ligation efficiency of T4 RNA Ligase 1 varies significantly for different substrates, following the order: ssRNA-ssRNA＞ssRNA-ssDNA＞ssDNA-ssDNA. Additionally, T4 RNA Ligase 1 can also be used for ligation between RNA and mononucleotides, provided that the mononucleotides are phosphorylated at both the 5' and 3' ends. This application is commonly used for 3'-end labeling of RNA.
Components
|
Component |
EG25204S |
EG25204M |
|
T4 RNA Ligase 1 (10 U/μl) |
100 μl |
500 μl |
| 10× T4 Rnl1 Buffer | 1.5 ml | 1.5 ml |
| 50% PEG 8000 (RNase-free) | 1 ml | 2×1 ml |
| ATP (10 mM ) | 200 μl | 1 ml |
Storage Condition
Store at -20℃ for 2 years.
