PRODUCT
Sgel
REF No.：
- EG21501S
Specs：
250 U
Price：
$42
Cut site：
m⁵CNNG(9/13)
5'...m⁵ C N N G(N)₉ ↓...3'
3'... G N N C(N)₁₃ ↑...5'*
*SgeI recognizes and cleaves DNA target sequences containing 5-mC sites, and can be recognized by either one-strand or double-strand methylation.
5'...m⁵ C N N G(N)₉ ↓...3'
3'... G N N C(N)₁₃ ↑...5'*
*SgeI recognizes and cleaves DNA target sequences containing 5-mC sites, and can be recognized by either one-strand or double-strand methylation.
Description
SgeI is a restriction endonuclease that cleaves DNA targets containing 5-methylcytosine on either single-stranded or double-stranded DNA. SgeI recognizes the m5CNNG(9/13)^ site and exhibits optimal cleavage in its unique buffer at 37°C. To ensure consistent performance, the enzyme's storage buffer contains pre-mixed BSA, which enhances enzyme stability and binds to potential contaminants that may be present in DNA preparations.
Components
|
Component |
Amount |
|
Sgel (5U/μl) |
50 μl |
|
10×Sgel Buffer |
1 ml |
Features and Usage
Recommended Reaction Conditions
1× Sgel Buffer；
Incubate at 37℃；
Refer to "Protocol for DNA Digestion" for reaction setup.
Heat Inactivation
Incubation at 80℃ for 20 minutes.
Storage Condition
-20℃
