PRODUCT
BspQI
REF No.：
- EG23503S
Specs：
500 U
Price：
$42
Isoschizomers：
SapI, LguI, PciSI
Cut site：
GCTCTTC(1/4)
5'...G C T C T T C (N)₁↓...3'
3'...C G A G A A G (N)₄↑...5'
5'...G C T C T T C (N)₁↓...3'
3'...C G A G A A G (N)₄↑...5'
Description
BspQI is a Type IIS restriction enzyme that recognizes non-palindromic sequences and cuts outside the recognition site. It is commonly used in Golden Gate assembly. The reaction buffer has been optimized to maximize the functionality of BspQI. Additionally, the reaction buffer contains recombinant albumin, which enhances the stability of various enzymes.
Components
|
Component |
Amount |
|
BspQI (10 U/μl) |
50 μl |
|
10× HN Buffer |
1 ml |
Features and Usage
Recommended Reaction Conditions
1× HN Buffer；
Incubate at 50℃；
Refer to "Protocol for DNA Digestion" for reaction setup.
Heat Inactivation
Incubation at 80℃ for 20 minutes.
Storage Condition
-20℃
