PciI is a Type IIP restriction endonuclease derived from Planococcus citreus, obtained through purification following recombinant expression in Escherichia coli. PciI uses the specialized reaction buffer, Cut Buffer C, enabling rapid digestion. Additionally, it is compatible with CutOne® buffer for double digestion, but star activity may result from extended digestion.

Recommended Reaction Conditions

1× Cut Buffer C；
Incubate at 37℃；
Refer to "Protocol for DNA Digestion" for reaction setup.

Heat Inactivation

Incubation at 80℃ for 20 minutes.

Methylation sensitivity

Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by the EcoKI methylase.

Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by the EcoBI methylase.

Store at -20℃ for 2 years.