PRODUCT
SgrAI
- EG25513S
5'...C R ↓ C C G G Y G...3'
3'...G Y G G C C ↑ R C...5'
Description
SgrAI is derived from Streptomyces griseus and is obtained through purification following recombinant expression in Escherichia coli. It specifically recognizes and cleaves the CR/CCGGYG sequence. SgrAI belongs to the non-LightNing® restriction enzymes; however, it is compatible with CutOne® buffer, allowing for double digestion with other LightNing® restriction enzymes. Due to its specificity, SgrAI requires two or more restriction sites to achieve efficient cleavage, with significantly reduced cleavage efficiency for substrates containing a single site.
Components
|
Component |
Amount |
|
SgrAI (10 U/μl) |
50 μl |
|
10× Cut Buffer B |
1 ml |
Features and Usage
Recommended Reaction Conditions
1× Cut Buffer B；
Incubate at 37℃；
Refer to "Protocol for DNA Digestion" for reaction setup.
Heat Inactivation
Incubation at 80℃ for 20 minutes.
Methylation sensitivity
Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by the CpG methylase.
Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by the EcoKI methylase.
Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by the EcoBI methylase.
