ApeKI is a Type IIP restriction enzyme derived from the Aeropyrum pernix K1. It recognizes and cleaves the sequence G/CWGC, generating sticky-end with a 3-base overhang. ApeKI is one of the preferred enzymes for Genotyping-by-Sequencing (GBS) and is widely used in plant and animal genetics, population evolution studies, and functional gene mapping.

Recommended Reaction Conditions

1× Cut Buffer C;
Incubate at 75℃;
Refer to "Protocol for DNA Digestion" for reaction setup.

Heat Inactivation

This enzyme can not be heat inactivated. Please purify the digested product by phenol/chloroform treatment or column-based purification kit.

Methylation sensitivity

Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by the CpG methylase.

Store at -20℃ for 2 years.