PRODUCT
BspEI
- EG25528S
5'...T↓C C G G A...3'
3'...A G G C C↑T...5'
Description
BspEI is a Type IIP restriction enzyme derived from Bacillus and obtained by recombinant expression and purification in Escherichia coli. BspEI recognizes and cleaves the sequence TCCGGA, generating a 4-base 5' overhang. BspEI requires the dedicated Cut Buffer C and is incompatible with the universal CutOne® Buffer.
Components
|
Component |
Amount |
|
BspEI (10 U/μl) |
50 μl |
|
10× Cut Buffer C |
1 ml |
Features and Usage
Recommended Reaction Conditions
1× Cut Buffer C；
Incubate at 37℃；
Refer to "Protocol for DNA Digestion" for reaction setup.
Heat Inactivation
Incubation at 80℃ for 20 minutes.
Methylation sensitivity
Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by the Dam methylase.
Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by the CpG methylase.
Storage Condition
Store at -20℃ for 2 years.
