PRODUCT
Description
M-MLV GIII Reverse Transcriptase is third-generation reverse transcriptase derived through genetic engineering and recombinant technology from the Moloney Murine Leukemia Virus (M-MLV). This enzyme has been modified to eliminate the RNase H activity. Additionally, it exhibits significantly improved thermal stability(optimal reaction temperature:50 ℃). These greatly improve the specificity and length of the synthesized first-strand cDNA, allowing for the synthesis of cDNA fragments up to 12 kb. Moreover, M-MLV GIII Reverse Transcriptase shows enhanced tolerance to RNA secondary structures.
Components
| Component | Amount | 
| M-MLV GIII Reverse Transcriptase(200 U/μl) | 50 μl | 
| 5× M-MLV First Strand Buffer | 500 μl | 
| 0.1 M DTT | 100 μl | 
Storage Condition
-20℃


