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M-MLV GIII Reverse Transcriptase

REF No.:
  • EG15124S
Specs:
10000 U
Price:
$50

Description

       M-MLV GIII Reverse Transcriptase is third-generation reverse transcriptase derived through genetic engineering and recombinant technology from the Moloney Murine Leukemia Virus (M-MLV). This enzyme has been modified to eliminate the RNase H activity. Additionally, it exhibits significantly improved thermal stability(optimal reaction temperature:50 ℃). These greatly improve the specificity and length of the synthesized first-strand cDNA, allowing for the synthesis of cDNA fragments up to 12 kb. Moreover, M-MLV GIII Reverse Transcriptase shows enhanced tolerance to RNA secondary structures.

Components

Component

Amount

M-MLV GIII Reverse Transcriptase(200 U/μl)

50 μl

5× M-MLV First Strand Buffer

500 μl

0.1 M DTT

100 μl

Storage Condition

-20℃

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