DNA Assembly Mix Mono is a kind of seamless assembly method, digest, ligate or end repair are not required. This method has been used to assemble different sizes of DNA fragments with varied overlaps (15~30 bp). And it offers a easy, fast and efficient DNA cloning technique.
       DNA Assembly Mix Mono is specifically designed for single-fragment DNA assembly. It can complete single-fragment recombination in as fast as 5 minutes with a positive rate of over 95%. Compared with DNA Assembly Mix Ultra (REF: EG24204), DNA Assembly Mix Mono does not rely on ligase, has simpler components, and is more compatible with unpurified PCR products. Meanwhile, DNA Assembly Mix Mono contains no ligase and relies on the repair system of E. coli, thus ensuring higher fidelity.

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