Q&A
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Q:
How I can transform or calculate reaction numbers in the unit of LightNing enzymes?
A:The concentration of LightNing enzymes is proprietary information. One rxn LightNingt enzyme activity is defined as: 1 µl of enzyme cleaves 1 µg of DNA substrate in 15 min at optimum temperature(most are 37℃) in 20 µl of 1× CutOne buffer.
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Q:
Do LightNing restriction enzymes contain BSA?
A:No，we have replaced BSA with rHSA，which is a well defined, animal-free recombinant human albumin.
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Q:
How many nucleotides do I have to add adjacent to the RE recognition site in order to get efficient cutting?
A:A minimum of 6 protected base pairs upstream and downstream of the cleavage site is generally required, with no specific sequence requirement.
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Q:
Do I have to set-up digests with LightNing® enzymes for 5-15 minutes? Can I digest longer?
A:LightNing® enzymes have the benefit of working fast (5-15 minutes), but are also designed and qualified to withstand longer（no more than 3h） digestions without degradation of DNA.
