BbvCI is derived from the BbvCI gene of Bacillus brevis (L. Ge) and is obtained through recombinant expression in Escherichia coli. BbvCI is a Type IIA restriction enzyme existing as a heterodimer composed of two distinct catalytic subunits, each with an independent cleavage site. It recognizes and cleaves the non-palindromic sequence CCTCAGC(-5/-2), generating 5' cohesive ends with 3-base overhangs. BbvCI uses CutOne® reaction buffer and is compatible with double digestion with other LightNing® restriction enzymes. However,  it is advisable to avoid extended digestion (exceeding 3 hours) to prevent star activity.

Recommended Reaction Conditions

1× CutOne® Buffer；
Incubate at 37℃；
Refer to "Protocol for DNA Digestion" for reaction setup.

Heat Inactivation

Incubation at 80℃ for 20 minutes.

Methylation sensitivity

Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by the CpG methylase.

-20℃