PRODUCT
SspDI (KasI)
REF No.：
- EG24514S
Specs：
250 U
Price：
$42
Isoschizomers：
KasI
Neoschizomer：
DinI, EgeI, EheI, Mly113I, NarI, PluTI, SfoI
Cut site：
GGCGCC
5'...G ↓ G C G C C...3'
3'...C C G C G ↑ G...5'
5'...G ↓ G C G C C...3'
3'...C C G C G ↑ G...5'
Description
SspDl belongs to the regular restriction enzyme series and recognizes the G^GCGCC sequence. Unlike the LightNing Restriction Endonucleases, SspDl requires a longer incubation time for complete digestion of the DNA substrate. However, this enzyme can still be used with Universal Cutone reaction buffer to achieve double digestion.
Components
|
Component |
Amount |
|
SspDI (10 U/μl) |
25 μl |
|
10× CutOne® Buffer |
1 ml |
|
10× CutOne® Color Buffer |
1 ml |
Features and Usage
Recommended Reaction Conditions
1× CutOne® Buffer；
Incubate at 37℃；
Refer to "Protocol for DNA Digestion" for reaction setup.
Heat Inactivation
Incubation at 80℃ for 20 minutes.
Methylation sensitivity
Cleavage with this restriction enzyme may be blocked or impaired when the substrate DNA is methylated by the CpG methylase.
Storage Condition
-20℃
